If desired, the data set can be viewed and rotated in 3D as well.īasically, focus stacking can also be accomplished without a motorised drive, since image stacks can also be produced by steadily moving the Z-drive (height) and operating the camera’s shutter release manually. Once shooting is complete, the image stack is post-processed by selecting Helicon Focus in the menu to combine the individual frames into a fully focused image.ĭepending on the number of frames and the processing speed of the computer, this consolidation process may take several minutes. The procedure can be monitored on the computer, and the frames captured are stored automatically on the PC. Depending on the specimen and magnification selected, up to 100 frames or more are taken. Shooting begins simply by clicking the start button. The software also supports remote selection of exposure brackets. The camera and the StackShot focusing rail are controlled remotely through the Helicon Remote software, which receives the Live View image from the camera via USB cable. The LM lens unit is moved with the StackShot drive. Some of our most commonly used tools include the StackShot system from Cognisys and Helicon software. Our LM photography solutions ( LM macroscopes and LM photo microscopes) are ideally suited for use with motorised focusing rails. The individual frames are then blended to produce a single image with a very high depth of field and resolution. For instance, while the depth of field at a microscope magnification of 100x is only a few µm, focus stacking overcomes this by taking multiple frames (image stacks) at different focus points. This image processing technique is commonly used in digital photomacrography or photomicrography to compensate for depth of field loss – a problem inherent to both areas of photography, since the depth of field shrinks due to the intense magnification required. Focus stacking with our LM macroscopes and LM photo microscopes Focus stacking is a method used to create images with enhanced depth of field.
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